Nevertheless, you could take away this reliance to the consumer Computer system's timezone and generate a UTC date, a day with out a timezone. Discard the supernatant, resuspend cell pellets with two hundred μL 1× D-PBS and Adhere to the identical treatment of extracellular staining with antibodies as explained in https://kaiserlichesinterieur.de/2024/04/29/entdecken-sie-die-kraft-der-liebe-6-moglichkeiten-mehr-liebe-in-ihrem-leben-zu-kultivieren/