Isolation of pure culture. Restreak poisonous tradition in duplicate on egg yolk agar medium. Incubate 1 plate anaerobically at 35°C. Incubate 2nd plate aerobically at 35°C. If colonies normal of C. botulinum PS: thank you on the amazing clients who source me with their particular ahead of and afters illustrations https://dermalfillercosmestics.com/